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| Litaker, R. Wayne; Holland, William C.; Hardison, D. Ransom; Pisapia, Francesco; Hess, Philipp; Kibler, Steven R.; Tester, Patricia A.. |
| Dinoflagellate species belonging to the genera Gambierdiscus and Fukuyoa produce ciguatoxins (CTXs), potent neurotoxins that concentrate in fish causing ciguatera fish poisoning (CFP) in humans. While the structures and toxicities of ciguatoxins isolated from fish in the Pacific and Caribbean are known, there are few data on the variation in toxicity between and among species of Gambierdiscus and Fukuyoa. Quantifying the differences in species-specific toxicity is especially important to developing an effective cell-based risk assessment strategy for CFP. This study analyzed the ciguatoxicity of 33 strains representing seven Gambierdiscus and one Fukuyoa species using a cell based Neuro-2a cytotoxicity assay. All strains were isolated from either the... |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00407/51839/52439.pdf |
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| Brissard, Charline; Herrenknecht, Christine; Sechet, Veronique; Herve, Fabienne; Pisapia, Francesco; Harcouet, Jocelyn; Lemee, Rodolphe; Chomerat, Nicolas; Hess, Philipp; Amzil, Zouher. |
| Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis assay. Results obtained with these two methods were comparable, suggesting ovatoxins... |
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Palavras-chave: Ostreopsis cf. ovata; Palytoxin & ovatoxins; Culture; Seafood contamination; LC-MS/MS; Hemolysis assay; Mediterranean Sea. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31025/29428.pdf |
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| Pisapia, Francesco; Sibat, Manoella; Herrenknecht, Christine; Lhaute, Korian; Gaiani, Greta; Ferron, Pierre-jean; Fessard, Valerie; Fraga, Santiago; Nascimento, Silvia M.; Litaker, R. Wayne; Holland, William C.; Roullier, Catherine; Hess, Philipp. |
| Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from... |
| Tipo: Text |
Palavras-chave: Gambierdiscus excentricus; Maitotoxin-4; Bioguided fractionation; Size-exclusion chromatography (LH-20); Neuro-2a (N2a) assay; LC-HRMS/MS (Q-Tof 6550); LC-LRMS/MS (API4000 QTrap). |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00392/50320/51001.pdf |
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| Pisapia, Francesco; Holland, William C.; Hardison, D. Ransom; Litaker, R. Wayne; Fraga, Santiago; Nishimura, Tomohiro; Adachi, Masao; Lam Nguyen-ngoc,; Sechet, Veronique; Amzil, Zouher; Herrenknecht, Christine; Hess, Philipp. |
| Species in the epi-benthic dinoflagellate genus Gambierdiscus produce ciguatoxins (CTXs) and maitotoxins (MTXs), which are among the most potent marine toxins known. Consumption of fish contaminated with sufficient quantities of CTXs causes Ciguatera Fish Poisoning (CFP), the largest cause of non-bacterial food poisoning worldwide. Maitotoxins, which can be found in the digestive system of fish, could also contribute to CFP if such tissues are consumed. Recently, an increasing number of Gambierdiscus species have been identified; yet, little is known about the variation in toxicity among Gambierdiscus strains or species. This study is the first assessment of relative CTX- and MTX-toxicity of Gambierdiscus species from areas as widespread as the... |
| Tipo: Text |
Palavras-chave: Ciguatera fish poisoning; Gambierdiscus; Ciguatoxins; Maitotoxins; Neuro-2a assay; Erythrocyte lysis assay. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00374/48552/49411.pdf |
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